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pla blocking solution  (Millipore)


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    Millipore pla blocking solution
    Pla Blocking Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/pla+blocking+solution/pm37965896-429-7-10
    Average 90 stars, based on 1 article reviews
    pla blocking solution - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Proximity Ligation Assay:

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..

    Article Title: Protein Kinase D3 (PKD3) Requires Hsp90 for Stability and Promotion of Prostate Cancer Cell Migration
    Article Snippet: .. Wells were blocked with PLA Blocking solution (Sigma Aldrich, St. Louis, MO, USA) for 1 h, then primary antibodies diluted in PLA antibody diluent solution (Sigma Aldrich, St. Louis, MO, USA) were added and incubated overnight at 4 °C. .. Rabbit polyclonal anti-Akt1 antibody was from Sigma–Aldrich, mouse monoclonal anti-Hsp90 from Institute of Immunology Ltd. (Tokyo, Japan), and rabbit polyclonal anti-PKD2 and rabbit polyclonal anti-PKD3 antibodies from Atlas Antibodies (Bromma, Sweden).

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma‐Aldrich) overnight at 4°C then washed with PBS. .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma‐Aldrich) overnight at 4°C then washed with PBS.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma-Aldrich) overnight at 4°C then washed with PBS. .. PLA probes (antimouse minus DUO92004 and antirabbit plus DUO92002, Sigma-Aldrich) were incubated together in PLA blocking solution for 20 min then added on the coverslips for 1 h at 37°C then washed two times with buffer A (150 mM NaCl, 10 mM Tris, 0.5% Tween).

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..

    Blocking Assay:

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..

    Article Title: Protein Kinase D3 (PKD3) Requires Hsp90 for Stability and Promotion of Prostate Cancer Cell Migration
    Article Snippet: .. Wells were blocked with PLA Blocking solution (Sigma Aldrich, St. Louis, MO, USA) for 1 h, then primary antibodies diluted in PLA antibody diluent solution (Sigma Aldrich, St. Louis, MO, USA) were added and incubated overnight at 4 °C. .. Rabbit polyclonal anti-Akt1 antibody was from Sigma–Aldrich, mouse monoclonal anti-Hsp90 from Institute of Immunology Ltd. (Tokyo, Japan), and rabbit polyclonal anti-PKD2 and rabbit polyclonal anti-PKD3 antibodies from Atlas Antibodies (Bromma, Sweden).

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma‐Aldrich) overnight at 4°C then washed with PBS. .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma‐Aldrich) overnight at 4°C then washed with PBS.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma-Aldrich) overnight at 4°C then washed with PBS. .. PLA probes (antimouse minus DUO92004 and antirabbit plus DUO92002, Sigma-Aldrich) were incubated together in PLA blocking solution for 20 min then added on the coverslips for 1 h at 37°C then washed two times with buffer A (150 mM NaCl, 10 mM Tris, 0.5% Tween).

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..

    Incubation:

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..

    Article Title: Protein Kinase D3 (PKD3) Requires Hsp90 for Stability and Promotion of Prostate Cancer Cell Migration
    Article Snippet: .. Wells were blocked with PLA Blocking solution (Sigma Aldrich, St. Louis, MO, USA) for 1 h, then primary antibodies diluted in PLA antibody diluent solution (Sigma Aldrich, St. Louis, MO, USA) were added and incubated overnight at 4 °C. .. Rabbit polyclonal anti-Akt1 antibody was from Sigma–Aldrich, mouse monoclonal anti-Hsp90 from Institute of Immunology Ltd. (Tokyo, Japan), and rabbit polyclonal anti-PKD2 and rabbit polyclonal anti-PKD3 antibodies from Atlas Antibodies (Bromma, Sweden).

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma‐Aldrich) overnight at 4°C then washed with PBS. .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma‐Aldrich) overnight at 4°C then washed with PBS.

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma-Aldrich) overnight at 4°C then washed with PBS. .. PLA probes (antimouse minus DUO92004 and antirabbit plus DUO92002, Sigma-Aldrich) were incubated together in PLA blocking solution for 20 min then added on the coverslips for 1 h at 37°C then washed two times with buffer A (150 mM NaCl, 10 mM Tris, 0.5% Tween).

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..



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    pla blocking solution  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology pla blocking solution
    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Pla Blocking Solution, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/Polylactic+acid/pmc12235512-148-3-12
    Average 93 stars, based on 1 article reviews
    pla blocking solution - by Bioz Stars, 2026-10
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    pla blocking solution  (Millipore)
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    Millipore pla blocking solution
    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Pla Blocking Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/pla+blocking+solution/pm37965896-429-7-10
    Average 90 stars, based on 1 article reviews
    pla blocking solution - by Bioz Stars, 2026-10
    90/100 stars
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    blocking solution included pla kit (duolink situ probe  (Merck & Co)
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    Merck & Co blocking solution included pla kit (duolink situ probe
    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Blocking Solution Included Pla Kit (Duolink Situ Probe, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/duo92002/pm37604854-260-41-52
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    duolink pla blocking solution  (Millipore)
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    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Duolink Pla Blocking Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/duolink+pla/pm37480622-106-6-10
    Average 90 stars, based on 1 article reviews
    duolink pla blocking solution - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    blocking solution supplied pla kit  (Millipore)
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    Millipore blocking solution supplied pla kit
    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
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    Image Search Results


    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Journal: Nucleic Acids Research

    Article Title: BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions

    doi: 10.1093/nar/gkaf626

    Figure Lengend Snippet: Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Article Snippet: After incubation with PLA blocking solution, HaCaT cells were incubated with anti-XPC (Santa Cruz, 1:200 dilution) and anti-BTG3 (Lab raised, 1:100 dilution) antibodies overnight at 4°C with gentle rocking.

    Techniques: Fluorescence, Two Tailed Test, Immunoprecipitation, Irradiation, Membrane, Confocal Microscopy